遺伝子・分子生物学研究室
動物、植物、微生物の生命現象を分子レベルで調べ、特にこれら生体内での遺伝子および酵素の働きを解き明かすための研究を行います。生き物の固有の特徴となっているさまざまな形質は、細胞内にある染色体（遺伝子）に設計図が書かれています。しかし、これら遺伝子がどのように発現し制御されているのか、また遺伝子によって作られるタンパク質の機能はまだ分かっていないことが非常に多く残っています。私達の研究室では微生物をモデルとして、人と微生物の関わりをテーマにしています。医療関連分野と理工学分野の橋渡し役になる人材育成を目的としています。
研究内容
環境ホルモン分解酵素遺伝子を応用した環境修復技術の開発
人の腸内の乳酸菌が持つ免疫機能を高めるメカニズムの解明
その他
人の腸内の乳酸菌が持つ免疫機能を高めるメカニズムの解明
その他
部屋（内線）・機器
遺伝子･分子生物学実験室1 27号館3F 307
遺伝子･分子生物学実験室2 27号館3F 317 （内）6737
• 遺伝子解析システム（ALOKA）
• DNAシーケンサー（ABI）
• GC-MS（ヒューレットパッカード）
• PCR遺伝子増幅装置（Bio-Rad）
• リアルタイム-PCRシステム（ABI）
• 遺伝子導入システム（Bio-Rad）
• フレンチプレス（細胞破砕装置）（AMINCO）
• FPLC／Bio-Pilot酵素精製システム（アマシャム）
• 高速液体クロマトグラフィー（ウォーターズ）
• 振とう培養装置（タイテック）
• ゲルイメージアナライザー（アマシャム）
• 超遠心分離装置（ベックマン）
遺伝子･分子生物学実験室2 27号館3F 317 （内）6737
• 遺伝子解析システム（ALOKA）
• DNAシーケンサー（ABI）
• GC-MS（ヒューレットパッカード）
• PCR遺伝子増幅装置（Bio-Rad）
• リアルタイム-PCRシステム（ABI）
• 遺伝子導入システム（Bio-Rad）
• フレンチプレス（細胞破砕装置）（AMINCO）
• FPLC／Bio-Pilot酵素精製システム（アマシャム）
• 高速液体クロマトグラフィー（ウォーターズ）
• 振とう培養装置（タイテック）
• ゲルイメージアナライザー（アマシャム）
• 超遠心分離装置（ベックマン）
最近の主な研究成果
【学術雑誌論文】
Complete genome sequence of the thermophilic PCB degrader Geobacillus sp. JF8 (NBRC 109937) Masaki Shintani, Yoshiyuki Ohtsubo, Kohei Fukuda, Akira Hosoyama, Shoko Ohji, Atsushi Yamazoe, Nobuyuki Fujita, Yuji Nagata, Masataka Tsuda, Takashi Hatta, and Kazuhide Kimbara, Genome Announc. 2014
Complete Genome Sequence of Ralstonia pickettii DTP0602, a 2,4,6-Trichlorophenol Degrader. Yoshiyuki Ohtsubo, Nobuyuki Fujita, Yuji Nagata, Masataka Tsuda, Tomohiro Iwasaki, Takashi Hatta, Genome Announc. , 1(6) e00903-13 2013
The regulatory mechanism of 2,4,6-trichlorophenol catabolic operon expression by HadR in Ralstonia pickettii DTP0602. Hideto Torii, Azumi Machida, Hirofumi Hara, Takashi Hatta, and Noboru Takizawa,Microbiology April 2013 159:665-677
Analysis of two gene clusters involved in 2,4,6-trichlorophenol degradation by Ralstonia pickettii DTP0602. Takashi Hatta, Eiji Fujii, Biosci. Biotechnol. Biochem.;76 (5) 892-899 2012
Crystallization and preliminary crystallographic analysis of maganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from Bacillus sp. JF8, Miki Senda, Takashi Hatta, Kazuhide Kimbara and Toshiya Senda Acta Cryst. F66, 282–285, 2010
Effect of HadE on Chlorophenol Monooxygenase from Ralstonia pickettii. T.Hatta, M.Iwamoto, T.Yonehara and M.Shimura,Bull. Res. Inst. of Tech., Okayama Univ. of Sci., 25, 34-38, 2007
bph genes of the thermophilic PCB degrader, Bacillus sp. JF8: characterization of the divergent ring-hydroxylating dioxygenase and hydrolase genes upstream of the Mn-dependent BphC.Mukerjee-Dhar G., M.Shimura, D.Miyazawa, K.Kimbara and T.Hatta, Microbiology, 151, 4139-4151, 2005
Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence. Asada N, Yokoyama G, Kawamoto N, Norioka S, Hatta T. Biochem. Genet. Jun;41(5-6):151-163. 2003
Characterization of a Novel Thermostable Mn (II)-dependent 2,3-Dihydroxybiphenyl 1,2-Dioxygenase from a Polychlorinated Biphenyl- and Naphthalene-degrading Bacillus sp. JF8., T.Hatta, G.M.-Dhar, J.Damborsky, H.Kiyohara, K.Kimbara,J. Biol. Chem., 278 (24) 21483-21492, 2003
Crystal structure of 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from the Rhodococcus sp. strain RHA1 of the PCB degradation pathway. N. Nandhagopal, A. Yamada , T. Hatta , E. Masai , M. Fukuda, Y. Mitsui , T. Senda, J. Mol. Biol. 2001 309 (5) 1139-1151
【特許】
＜Europian Patent＞
曽我部敦、岡正則、稲垣賢二、八田貴、西瀬弘, 「新規なグリセロールキナーゼ、該遺伝子及び該を用いたグリセロールキナーゼの製造法」特開2004-121234, 2004
A. Sogabe, M. Oka, T. Hatta and K. Inagaki: Novel Glycerol Kinase, Gene Thereof and Process for Process for Producing the Glycerol Kinase by Using the Gege.? 1538211（登録2009.03.04）欧州
A. Sogabe, M. Oka, T. Hatta and K. Inagaki: Glycerol kinase, which has high resistance against preservative. 7618800（登録2009.11.17）米国
【国際会議発表】
Takashi Hatta, Suguru Tanaka, and Hirofumi Hara: 3-Oxoadipate Degradation Gene Clusters in 2,4,6-Trichlorophenol-Degrading Ralstonia pickettii DTP0602.15th International Biotechnology Symposium and Exhibition (IBS 2012) (Daegu, Korea) (2012)9.16-21
Hideto Torii, Hirofumi Hara, Hirotaka Yabe, Noboru Takizawa, and Takashi Hatta : Regulatory System of 2,4,6-Trichlorophenol Degradation Genes Expression inc Ralstonia pickettii Strain DTP0602. The 4th Congress of European Microbiologists, FEMS 2011 (Geneva, Switzerland) (2011) June 26-30
T.Hatta, T.Yonehara, M.Iwamoto and N.Takizawa, Characterization of chlorophenol monooxygenase from 2,4,6-trichlorophenol degrader Ralstonia pickettii DTP 0602, 8th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Leipzig, Germany), 2006
Y.Akai, M.Senda, K.Sugimoto, T.Hatta, K.Kimbara, M.Fukuda and Toshiya Senda, Crystal structures of extradiol type dioxygenases – Comparison of reaction intermediate structures and their implications, The 14th Takeda Science Foundation Symposium on Bioscience The 50th Anniversary of Oxygenases – Advances and Reflections – (Kyoto, Japan), 2006
T.Hatta, S.Mabuchi, H.Ito, T.Yonehara, S.Kusuda, H.Kiyohara and N.Takizawa, Regulatory gene involved in the degradation of 2,4,6-trichlorophenol in Ralstonia pickettii DTP0602, 7th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Chicago, USA), 2004
Complete genome sequence of the thermophilic PCB degrader Geobacillus sp. JF8 (NBRC 109937) Masaki Shintani, Yoshiyuki Ohtsubo, Kohei Fukuda, Akira Hosoyama, Shoko Ohji, Atsushi Yamazoe, Nobuyuki Fujita, Yuji Nagata, Masataka Tsuda, Takashi Hatta, and Kazuhide Kimbara, Genome Announc. 2014
Complete Genome Sequence of Ralstonia pickettii DTP0602, a 2,4,6-Trichlorophenol Degrader. Yoshiyuki Ohtsubo, Nobuyuki Fujita, Yuji Nagata, Masataka Tsuda, Tomohiro Iwasaki, Takashi Hatta, Genome Announc. , 1(6) e00903-13 2013
The regulatory mechanism of 2,4,6-trichlorophenol catabolic operon expression by HadR in Ralstonia pickettii DTP0602. Hideto Torii, Azumi Machida, Hirofumi Hara, Takashi Hatta, and Noboru Takizawa,Microbiology April 2013 159:665-677
Analysis of two gene clusters involved in 2,4,6-trichlorophenol degradation by Ralstonia pickettii DTP0602. Takashi Hatta, Eiji Fujii, Biosci. Biotechnol. Biochem.;76 (5) 892-899 2012
Crystallization and preliminary crystallographic analysis of maganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from Bacillus sp. JF8, Miki Senda, Takashi Hatta, Kazuhide Kimbara and Toshiya Senda Acta Cryst. F66, 282–285, 2010
Effect of HadE on Chlorophenol Monooxygenase from Ralstonia pickettii. T.Hatta, M.Iwamoto, T.Yonehara and M.Shimura,Bull. Res. Inst. of Tech., Okayama Univ. of Sci., 25, 34-38, 2007
bph genes of the thermophilic PCB degrader, Bacillus sp. JF8: characterization of the divergent ring-hydroxylating dioxygenase and hydrolase genes upstream of the Mn-dependent BphC.Mukerjee-Dhar G., M.Shimura, D.Miyazawa, K.Kimbara and T.Hatta, Microbiology, 151, 4139-4151, 2005
Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence. Asada N, Yokoyama G, Kawamoto N, Norioka S, Hatta T. Biochem. Genet. Jun;41(5-6):151-163. 2003
Characterization of a Novel Thermostable Mn (II)-dependent 2,3-Dihydroxybiphenyl 1,2-Dioxygenase from a Polychlorinated Biphenyl- and Naphthalene-degrading Bacillus sp. JF8., T.Hatta, G.M.-Dhar, J.Damborsky, H.Kiyohara, K.Kimbara,J. Biol. Chem., 278 (24) 21483-21492, 2003
Crystal structure of 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from the Rhodococcus sp. strain RHA1 of the PCB degradation pathway. N. Nandhagopal, A. Yamada , T. Hatta , E. Masai , M. Fukuda, Y. Mitsui , T. Senda, J. Mol. Biol. 2001 309 (5) 1139-1151
【特許】
＜Europian Patent＞
曽我部敦、岡正則、稲垣賢二、八田貴、西瀬弘, 「新規なグリセロールキナーゼ、該遺伝子及び該を用いたグリセロールキナーゼの製造法」特開2004-121234, 2004
A. Sogabe, M. Oka, T. Hatta and K. Inagaki: Novel Glycerol Kinase, Gene Thereof and Process for Process for Producing the Glycerol Kinase by Using the Gege.? 1538211（登録2009.03.04）欧州
A. Sogabe, M. Oka, T. Hatta and K. Inagaki: Glycerol kinase, which has high resistance against preservative. 7618800（登録2009.11.17）米国
【国際会議発表】
Takashi Hatta, Suguru Tanaka, and Hirofumi Hara: 3-Oxoadipate Degradation Gene Clusters in 2,4,6-Trichlorophenol-Degrading Ralstonia pickettii DTP0602.15th International Biotechnology Symposium and Exhibition (IBS 2012) (Daegu, Korea) (2012)9.16-21
Hideto Torii, Hirofumi Hara, Hirotaka Yabe, Noboru Takizawa, and Takashi Hatta : Regulatory System of 2,4,6-Trichlorophenol Degradation Genes Expression inc Ralstonia pickettii Strain DTP0602. The 4th Congress of European Microbiologists, FEMS 2011 (Geneva, Switzerland) (2011) June 26-30
T.Hatta, T.Yonehara, M.Iwamoto and N.Takizawa, Characterization of chlorophenol monooxygenase from 2,4,6-trichlorophenol degrader Ralstonia pickettii DTP 0602, 8th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Leipzig, Germany), 2006
Y.Akai, M.Senda, K.Sugimoto, T.Hatta, K.Kimbara, M.Fukuda and Toshiya Senda, Crystal structures of extradiol type dioxygenases – Comparison of reaction intermediate structures and their implications, The 14th Takeda Science Foundation Symposium on Bioscience The 50th Anniversary of Oxygenases – Advances and Reflections – (Kyoto, Japan), 2006
T.Hatta, S.Mabuchi, H.Ito, T.Yonehara, S.Kusuda, H.Kiyohara and N.Takizawa, Regulatory gene involved in the degradation of 2,4,6-trichlorophenol in Ralstonia pickettii DTP0602, 7th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Chicago, USA), 2004