Genetic Engineering Lab., BME, OUS

Introduction

　Our laboratory aim for elucidating the physiology of animals, plants, and bacteria under molecular level, especially “Gene” and “Protein” function on these organisms. The inherent characters of each organism are encoded by “Chromosome (or Genes)” in cell nucleus. However, we still don’t know a lot about how genes are regulated and expressed, and what the function of the　gene products (proteins) are. We are concerned about “Between human and bacteria”, and use bacteria as a model organism.

Researchs

Characterization of genes and proteins for synthesis antibiotics and anticancer drug.
Development of clean-up technology of environmental pollutant by its degrading bacterial genes and proteins.
Elucidate the mechanism of enhanced immunological function on lactic acid　 bacteria.

Members

Professor Takashi Hatta,

27# 3F,　thatta＠bme．ous．ac．jp，Tel: +81-86-256-9515

Room & Devices

Papers (Last five years)

【Major Publications】

T.Hatta, M.Iwamoto, T.Yonehara and M.Shimura, Effect of HadE on Chlorophenol Monooxygenase from Ralstonia pickettii. Bull. Res. Inst. of Tech., Okayama Univ. of Sci., 25, 34-38, 2007
E.Masai, Y.Yamamoto, T.Inoue, K.Takamura, H.Hara, D.Kasai, Y.Katayama and M.Fukuda, Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6. Biosci, Biotech, and Biochem. 71:2487-2492, 2007
van der Geize, R., K.Yam, T.Heuser, M.H.Wilbrink, H.Hara, W.C.Anderton, E.Sim, L.Dijkhuizen, J.E.Davies, W.W.Mohn and L.D.Eltis, A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages, Proc. Natl. Acad. Sci. USA, 104:1947-1952, 2007
H.Hara, L.D.Eltis, J.E.Davies and W.W.Mohn, Transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in Rhodococcus sp. RHA1. J. Bacteriol, 72: 6183-93, 2006
McLeod,M.P., R.L.Warren, N.Araki, W.W.L.Hsiao, M.Myhre, C.Fernandes, D.Miyazawa, W.Wong, A.L.Lillquist, D.Wang, M.Dosanjh, H.Hara, A.Petrescu, R.D.Morin, G.Yang, J.M.Stott, J.E.Schein, H.Shin, D.Smailus, A.S.Siddiqui, M.A.Marra, S.J.M.Jones, R.Holt, F.S.L.Brinkman, K.Miyauchi, M.Fukuda, J.E.Davies, W.W.Mohn and L.D.Eltis, The complete genome of Rhodococcus sp. RHA1: insights into a catabolic powerhouse, Proc. Natl. Acad. Sci. USA, 103:15582-15587, 2006
Goncalves,E.R., H.Hara, D.Miyazawa, J.E.Davies, L.D.Eltis, W.W.Mohn, Transcriptomic assessment of isozymes in the biphenyl pathway of Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol, 72: 6183-93, 2006.
Sasoh,M., E.Masai, S.Ishibashi, H.Hara, N.Kamimura, K.Miyauchi, M.Fukuda, Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Appl. Environ. Microbiol, 72:1825-32, 2006
Mukerjee-Dhar G., M.Shimura, D.Miyazawa, K.Kimbara and T.Hatta, bph genes of the thermophilic PCB degrader, Bacillus sp. JF8: characterization of the divergent ring-hydroxylating dioxygenase and hydrolase genes upstream of the Mn-dependent BphC. Microbiology, 151, 4139-4151, 2005
D.Miyazawa, G.Mukerjee-Dhar, M.Shimura, T.Hatta and K.Kimbara, Genes for Mn (II)-dependent NahC and Fe (II)-dependent NahH located in close proximity in the thermophilic naphthalene and PCB degrader, Bacillus sp. JF8: cloning and characterization. Microbiology, 150, (4) 150: 993-1004, 2004.
T.Hatta, G.M.-Dhar, J.Damborsky, H.Kiyohara, K.Kimbara, Characterization of a Novel Thermostable Mn (II)-dependent 2,3-Dihydroxybiphenyl 1,2-Dioxygenase from a Polychlorinated Biphenyl- and Naphthalene-degrading Bacillus sp. JF8. J. Biol. Chem., 278 (24) 21483-21492, 2003
H.Hara, E.Masai, K.Miyauchi, Y.Katayama and M.Fukuda, Characterization of 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6. J. Bacteriol, 185:41-50, 2003
N.Asada, G.Yokoyama, N.Kawamoto, S.Norioka, T.Hatta, Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence. Biochem Genet, 41 (5-6): 151-163, 2003

【Patents】

A.Sogabe, M.Okabe, K.Inagaki and T.Hatta, Novel Glycerol Kinase, Gene Thereof and Process for Process for Producing the Glycerol Kinase by Using the Gege. Patent No. 03795305.6-2401-JP0311411, 2006

【International Conference】

T.Hatta, T.Yonehara, M.Iwamoto and N.Takizawa, Characterization of chlorophenol monooxygenase from 2,4,6-trichlorophenol degrader Ralstonia pickettii DTP 0602, 8th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Leipzig, Germany), 2006
Y.Akai, M.Senda, K.Sugimoto, T.Hatta, K.Kimbara, M.Fukuda and Toshiya Senda, Crystal structures of extradiol type dioxygenases - Comparison of reaction intermediate structures and their implications, The 14th Takeda Science Foundation Symposium on Bioscience The 50th Anniversary of Oxygenases - Advances and Reflections - (Kyoto, Japan), 2006
H.Hara, E.R.Goncalves, Lindsay E. Eltis, Julian E. Davies, William W. Mohn, Transcriptomic analysis of aromatic compound biodegradation by Rhodococcus sp. RHA1, The 21st Century COE Program, The 6th International Symposium on“Global Renaissance by Green Energy Revolution”. (Nagaoka, Japan), 2006
H.Hara, Edmilson R.Goncalves, Lindsay E. Eltis, Julian E. avies, William W.Mohn, Transcriotome analysis of phthalate degradation by Rhodococcus sp. RHA1, GENEME 2005: International Conference on Microbial Genomes. (Falifax, Canada), 2005
Edmilson R. Goncalves, H.Hara, A.Pu, M.Fukuda, Lindsay Eltis, Julian Davies, William W.Mohn, Microarray analyis to elucidate multiple enzymes used for degradation of aromatic compounds by Rhodococcus sp. RHA1, ASM conference on Integrating Metabolism and Genomics (IMAGE). (Montreal, Canada), 2005
T.Hatta, S.Mabuchi, H.Ito, T.Yonehara, S.Kusuda, H.Kiyohara and N.Takizawa, Regulatory gene involved in the degradation of 2,4,6-trichlorophenol in Ralstonia pickettii DTP0602, 7th Biennial Symposium International Society of Environmental Boitechnol (ISEB) (Chicago, USA), 2004
H.Hara, Edmilson R. Goncalves, Aihua Pu, Lindsay Eltis, Julian Davies, William W.Mohn, Transcriptome analysis of aromatic-compounds degradation by Rhodococcus sp. RHA1, GENOME 2004: International Conference on the Analysis of Microbial and Other Genomes, (Cambridge, United Kingdom), 2004
T.Hatta, K.Suzuki, M.Shimura and K.Kimbara, Analysys of bph Gene Cluster of Psychrotrophic PCB/ Biphenyl-Degrading Burkholderia sp. MEG1 Pseudomonas 2003 (Qubec City, Canada), 2003
Edmilson R.Goncalves, H.Hara, Christine Florizone, Ritesh Patel, Matt Myhre, Manisha Dosanjh, M.Fukuda, Lindsay Eltis, Julian Davies, William Mohn, Transcriptome analysis of Rhodococcus sp. RHA1degrading phthalate and biphenyl, American Society for Microbiology Northwest Branch, (Vancouver, Canada), 2003